Identification and purification of a functional amine transporter from bovine chromaffin granules.

@article{SternBach1990IdentificationAP,
  title={Identification and purification of a functional amine transporter from bovine chromaffin granules.},
  author={Y Stern-Bach and N Greenberg-Ofrath and Ianco Flechner and Shimon Schuldiner},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 7},
  pages={3961-6}
}
The amine transporter from bovine chromaffin granules has been purified in a functional state. Two isoforms with different pI values have been separated and shown to be active. One with an unusually acidic pI (approximately 3.5) has been shown to be a glycoprotein with an apparent Mr of 80,000. The purified polypeptide catalyzes transport of serotonin upon reconstitution with an apparent Km of 2 microM and a Vmax of 140 nmol/mg/min, 150-200-fold higher than the one determined in the native… CONTINUE READING

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