Identification and preliminary characterization of temperature-sensitive mutations affecting HlyB, the translocator required for the secretion of haemolysin (HlyA) from Escherichia coli

@article{Blight1994IdentificationAP,
  title={Identification and preliminary characterization of temperature-sensitive mutations affecting HlyB, the translocator required for the secretion of haemolysin (HlyA) from Escherichia coli},
  author={Mark A Blight and Andr{\'e}a de Lima Pimenta and J. -C. Lazzaroni and Caroline Dando and Larissa Kotelevets and S. Seror and Ian Barry Holland},
  journal={Molecular and General Genetics MGG},
  year={1994},
  volume={245},
  pages={431-440}
}
We have carried out a genetic analysis of Escherichia coli HlyB using in vitro(hydroxylamine) mutagenesis and regionally directed mutagenesis. From random mutagenesis, three mutants, temperature sensitive (Ts) for secretion, were isolated and the DNA sequenced: Glyl0Arg close to the N-terminus, Gly408Asp in a highly conserved small periplasmic loop region PIV, and Pro624Leu in another highly conserved region, within the ATP-binding region. Despite the Ts character of the Gly10 substitution, a… CONTINUE READING
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