Identification and partial purification of a novel milk clotting enzyme from Onopordum turcicum

@article{Tamer2004IdentificationAP,
  title={Identification and partial purification of a novel milk clotting enzyme from Onopordum turcicum},
  author={I. Melih Tamer},
  journal={Biotechnology Letters},
  year={2004},
  volume={15},
  pages={427-432}
}
Seeds, flowers and leaves of Onopordum turcicum were found to contain proteolytic enzymes able to coagulate milk. Extraction, concentration and identification of the operational parameters affecting the activity of the enzyme complex were followed by partial purification steps involving gel-filtration and ion-exchange chromatography. Milk clotting activity of the enzyme complex was tested in several steps of its purification and an increase of almost 200 fold was obtained. Molecular weight of… CONTINUE READING

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