Identification and organization of carbon dioxide fixation genes in Xanthobacter flavus H4-14


The genes encoding the large (cfxL) and small (cfxS) subunits of ribulose-1,5-bisphosphate carboxylase (RuBisC/O) from Xanthobacter favus H4-14 were identified and characterized. The RuBisC/O genes are separated by 11 by and cotranscribed in Escherichia coli from the lac promoter in the order cfxLS. Primer extension and R-loop experiments with RNA isolated from autotrophically grown X. favus H4-14 showed that transcription of cfxL and cfxS initiated 22 by upstream from cfxL and resulted in a mRNA of at least 2.3 kb. DNA sequence analysis identified the start of an open reading frame transcribed divergently from cfxL, and displaying significant similarities with genes belonging to the lysR family of transcriptional activators. Downstream from cfxS an additional open reading frame was identified with unknown function. Expression studies showed that the genes encoding fructosebisphosphatase (cfxF) and phosphoribulokinase (cfxP) are located downstream from cfxLS. The cfxF and cfxP genes are cotranscribed in the same direction as cfxLS in the order cfxFP.

DOI: 10.1007/BF00269865

7 Figures and Tables


Citations per Year

608 Citations

Semantic Scholar estimates that this publication has 608 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Meijer1991IdentificationAO, title={Identification and organization of carbon dioxide fixation genes in Xanthobacter flavus H4-14}, author={Wim G. Meijer and A. C. Arnberg and Hans G. Enequist and Peter Terpstra and Mary E. Lidstrom and Lubbert Dijkhuizen}, journal={Molecular and General Genetics MGG}, year={1991}, volume={225}, pages={320-330} }