Identification and mutagenesis by allelic exchange of choE, encoding a cholesterol oxidase from the intracellular pathogen Rhodococcus equi.

@article{Navas2001IdentificationAM,
  title={Identification and mutagenesis by allelic exchange of choE, encoding a cholesterol oxidase from the intracellular pathogen Rhodococcus equi.},
  author={Javier Navas and B. Gonz{\'a}lez-zorn and N{\'e}stor Ladr{\'o}n and P. L. Romera Garrido and Jos{\'e} A V{\'a}zquez-Boland},
  journal={Journal of bacteriology},
  year={2001},
  volume={183 16},
  pages={
          4796-805
        }
}
The virulence mechanisms of the facultative intracellular parasite Rhodococcus equi remain largely unknown. Among the candidate virulence factors of this pathogenic actinomycete is a secreted cholesterol oxidase, a putative membrane-damaging toxin. We identified and characterized the gene encoding this enzyme, the choE monocistron. Its protein product, ChoE, is homologous to other secreted cholesterol oxidases identified in Brevibacterium sterolicum and Streptomyces spp. ChoE also exhibits… CONTINUE READING

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