Identification and molecular evolution of new dynein-like protein sequences in rat brain.

@article{Tanaka1995IdentificationAM,
  title={Identification and molecular evolution of new dynein-like protein sequences in rat brain.},
  author={Yoshiya Tanaka and Zhi-gang Zhang and Nobutaka Hirokawa},
  journal={Journal of cell science},
  year={1995},
  volume={108 ( Pt 5)},
  pages={1883-93}
}
RT-PCR cloning was performed to find unknown members of the dynein superfamily expressed in rat brain. Six kinds of degenerate primers designed for the dynein catalytic domain consensuses were used for extensive PCR amplifications. We have sequenced 550 plasmid clones which turned out to include 13 kinds of new dynein-like sequences (DLP1-8, 9A/B, 10-12) and cytoplasmic dynein heavy chain. In these clones, alternative splicing was detected for a 105 nt-domain containing the CFDEFNRI consensus… CONTINUE READING
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Molecular Cloning: a Laboratory Manual, 2nd edn

  • J. Sambrook, E. F. Fritsch, T. Maniatis
  • 1989
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