Identification and mapping of polymorphic SSR markers from expressed gene sequences of barley and wheat

@article{Holton2004IdentificationAM,
  title={Identification and mapping of polymorphic SSR markers from expressed gene sequences of barley and wheat},
  author={Timothy A. Holton and John T. Christopher and Linda McClure and Natalie Harker and Robert J Henry},
  journal={Molecular Breeding},
  year={2004},
  volume={9},
  pages={63-71}
}
The growing availability of EST sequences from a range of crop plantsprovides a potentially valuable source of new DNA markers. We have examined theInternational Triticeae EST Cooperative database for the presence ofdinucleotide and trinucleotide simple sequence repeats. Analysis of 24,344 ESTsidentified 388 dinucleotide repeats and 978 trinucleotide repeats in ESTs,representing 1.6% and 4.0% of the total number of ESTs, respectively. To testthe utility and cross-species transferability of EST… CONTINUE READING
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