Identification and localization of BK-beta subunits in the distal nephron of the mouse kidney.

@article{Grimm2007IdentificationAL,
  title={Identification and localization of BK-beta subunits in the distal nephron of the mouse kidney.},
  author={P Richard Grimm and Ruth M Foutz and Robert Brenner and Steven C. Sansom},
  journal={American journal of physiology. Renal physiology},
  year={2007},
  volume={293 1},
  pages={F350-9}
}
Large-conductance, Ca(2+)-activated K(+) channels (BK), comprised of pore-forming alpha- and accessory beta-subunits, secrete K(+) in the distal nephron under high-flow and high-K(+) diet conditions. BK channels are detected by electrophysiology in many nephron segments; however, the accessory beta-subunit associated with these channels has not been determined. We performed RT-PCR, Western blotting, and immunohistochemical staining to determine whether BK-beta1 is localized to the connecting… CONTINUE READING