Identification and initial characterization of translational initiation factor 2 from bovine mitochondria.

@article{Liao1990IdentificationAI,
  title={Identification and initial characterization of translational initiation factor 2 from bovine mitochondria.},
  author={Huaxin Liao and Linda L. Spremulli},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 23},
  pages={
          13618-22
        }
}
The bovine liver mitochondrial factor that promotes the binding of fMet-tRNA to mitochondrial ribosomes, initiation factor 2 (IF-2mt), has been identified in the postribosomal supernatant fraction of isolated liver mitochondria. This factor has been purified approximately 5,000-fold and present preparations are estimated to be about 10% pure. IF-2mt has an apparent molecular weight of about 140,000 as determined by gel filtration chromatography. IF-2mt is active in stimulating fMet-tRNA binding… Expand
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