Identification and functional characterization of the NanH extracellular sialidase from Corynebacterium diphtheriae.

@article{Kim2010IdentificationAF,
  title={Identification and functional characterization of the NanH extracellular sialidase from Corynebacterium diphtheriae.},
  author={Seonghun Kim and Doo-Byoung Oh and Ohsuk Kwon and Hyun Kang},
  journal={Journal of biochemistry},
  year={2010},
  volume={147 4},
  pages={
          523-33
        }
}
Corynebacterium diphtheriae, a pathogenic Gram-positive bacterium, contains sialic acids on its cell surface, but no genes related to sialic acid decoration or metabolism have been reported in C. diphtheriae. In the present study, we have identified a putative sialidase gene, nanH, from C. diphtheriae KCTC3075 and characterized its product for enzyme activity. Interestingly, the recombinant NanH protein was secreted as a catalytically active sialidase into the periplasmic space in Escherichia… CONTINUE READING
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