Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris.

@article{Ehrlich2013IdentificationAF,
  title={Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris.},
  author={Hermann Ehrlich and Oksana V. Kaluzhnaya and Eike Brunner and Mikhail V Tsurkan and Alexander V. Ereskovsky and Micha Ilan and Konstantin R. Tabachnick and Vasilii V. Bazhenov and Silvia Paasch and Martin Kammer and Ren{\'e} Born and Allison Stelling and Roberta Galli and Sergei I. Belikov and Olga V Petrova and Victor V Sivkov and Denis V Vyalikh and Sebastian Hunoldt and Gert W{\"o}rheide},
  journal={Journal of structural biology},
  year={2013},
  volume={183 3},
  pages={474-483}
}
This work demonstrates that chitin is an important structural component within the skeletal fibers of the freshwater sponge Spongilla lacustris. Using a variety of analytical techniques ((13)C solid state NMR, FT-IR, Raman, NEXAFS, ESI-MS, Morgan-Elson assay and Calcofluor White Staining); we show that this sponge chitin is much closer to α-chitin, known to be present in other animals, than to β-chitin. Genetic analysis confirmed the presence of chitin synthases, which are described for the… CONTINUE READING
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