Identification and expression of the Escherichia coli fdhD and fdhE genes, which are involved in the formation of respiratory formate dehydrogenase

@article{Schlindwein1990IdentificationAE,
  title={Identification and expression of the Escherichia coli fdhD and fdhE genes, which are involved in the formation of respiratory formate dehydrogenase},
  author={Carolina Casco Duarte Schlindwein and Gérard Giordano and C L Santini and M A Mandrand},
  journal={Journal of Bacteriology},
  year={1990},
  volume={172},
  pages={6112 - 6121}
}
The two closely linked fdhD and fdhE genes of Escherichia coli are required for the formation of active membrane-bound phenazine methosulfate-linked formate dehydrogenase (FDH-PMS). Both genes were isolated from a cosmid library. Restriction endonuclease analysis associated with Mu dII1734 insertion mutagenesis indicated that the two genes were separated by at least 4 kilobases and transcribed in opposite orientations. Initial experiments indicate that the region between the two genes seems not… 
Expression and characterization of the Escherichia coli fdo locus and a possible physiological role for aerobic formate dehydrogenase
TLDR
Aerobically expressed FDH-Z and NAR-Z enzymes were shown to reduce nitrate at the expense of formate under anaerobic conditions, suggesting that this pathway would allow the cell to respond quickly to anaerobiosis.
Genetic evidence that genes fdhD and fdhE do not control synthesis of formate dehydrogenase-N in Escherichia coli K-12
TLDR
These complementation tests indicate that the S. typhimurium fdnC and fdnB genes are functionally homologous to the E. coli fdhD and fdhE genes, respectively, indicating that the rha-linked Salmonella typhIMurium genes are required primarily for formate dehydrogenase-N activity.
Expression and operon structure of the sel genes of Escherichia coli and identification of a third selenium-containing formate dehydrogenase isoenzyme
TLDR
A detailed analysis of the expression of the sel genes showed that transcription was constitutive, being influenced neither by aerobiosis or anaerobiosis nor by the intracellular selenium concentration, and it is probable that this selenopolypeptide constitutes a major component of the formate oxidase.
Suppression of Escherichia coli formate hydrogenlyase activity by trimethylamine N-oxide is due to drainage of the inducer formate.
TLDR
Results strongly suggest that formate-dependent reduction of TMAO via FDH-N and TOR reduces the amount of formate available for induction of the formate hydrogenlyase pathway.
In vivo role of FdhD and FdmE in formate metabolism in Pseudomonas putida: Redundancy and expression in the stationary phase.
TLDR
Results suggest that redundancy in formaldehyde/formate metabolism in P. putida extends to the proteins involved in maturation/location of formate dehydrogenase complexes.
Biosynthesis of the respiratory formate dehydrogenases from Escherichia coli: characterization of the FdhE protein
TLDR
It is shown that E. coli FdhE interacts with the catalytic subunits of the respiratory formate dehydrogenases, and site-directed mutagenesis shows that conserved cysteine motifs are essential for the physiological activity of the Fdh E protein and are also involved in iron ligation.
A Sulfurtransferase Is Essential for Activity of Formate Dehydrogenases in Escherichia coli*
TLDR
FdhD is a sulfurtransferase between IscS and FdhF and is thereby essential to yield FDH activity, which is iron/molybdenum/selenium-containing enzymes.
A-Type Carrier Protein ErpA Is Essential for Formation of an Active Formate-Nitrate Respiratory Pathway in Escherichia coli K-12
TLDR
The findings suggest that ErpA and IscA have overlapping roles in assembly of these anaerobic respiratory enzymes but demonstrate that ErPA is essential for the production of active enzymes.
Involvement of formate dehydrogenases in stationary phase oxidative stress tolerance in Escherichia coli
TLDR
It is suggested that formate dehydrogenase‐O serves as an electron transfer element in glucose metabolism to promote oxidative stress tolerance and survival in stationary phase.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 36 REFERENCES
Escherichia coli genes whose products are involved in selenium metabolism
TLDR
The results strongly supports the notion that the pleiotropic fdh mutants analyzed possess a lesion in the gene(s) encoding the biosynthesis or the incorporation of selenocysteine in the formate dehydrogenase subunits of FDHN and FDHH.
Mutants of Escherichia coli specifically deficient in respiratory formate dehydrogenase activity.
TLDR
Construction of merodiploid strains harbouring various combinations of the mutated alleles, fdhE on the episome and fdhD on the chromosome, led to the restoration of FDH-PMS activity by complementation of the products encoded by the respective wild-type alleles.
Formate-nitrate respiration in Salmonella typhimurium: studies of two rha-linked fdn genes
TLDR
The results are consistent with two distinct rha-linked fdn genes, for which it is suggested using the designations fdnB (group I) and fdnC (group II).
Homology between CAP and Fnr, a regulator of anaerobic respiration in Escherichia coli.
The organization of formate dehydrogenase in the cytoplasmic membrane of Escherichia coli.
TLDR
A procedure involving agglutination with specific antibodies is described which appears to fractionate membrane vesicles of mixed orientation into two populations, one with the same membrane orientation as that of spheroplasts and the other opposite orientation.
Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci
TLDR
The properties of mutants of E. coli which are defective with respect to nitrate reductase activity were examined and it was concluded that the chlE and chlG loci are involved in the synthesis of insertion of molybdenum cofactor.
Biochemical and immunological evidence for a second nitrate reductase in Escherichia coli K12.
TLDR
It is shown that E. coli has two types of nitrate reductase, one of which is a membrane-bound molybdoenzyme able to couple formate oxidation with nitrate reduction and another which shares some similarities as well as differences with the known enzyme.
Marker-exchange mutagenesis of a pectate lyase isozyme gene in Erwinia chrysanthemi
TLDR
The evidence suggests that this isozyme is not necessary for soft-rot pathogenesis, as determined by activity-stained isoelectric-focusing polyacrylamide gels.
Nitrate Reductase Complex of Escherichia coli K-12: Participation of Specific Formate Dehydrogenase and Cytochrome b1 Components in Nitrate Reduction
TLDR
It is concluded that nitrate reduction in E. coli is mediated by the sequential operation of a specific formate dehydrogenase, two specific cytochrome b(1) components, and nitrate reductase.
...
1
2
3
4
...