Identification and expression analysis of cobia (Rachycentron canadum) Toll-like receptor 9 gene.

@article{Byadgi2014IdentificationAE,
  title={Identification and expression analysis of cobia (Rachycentron canadum) Toll-like receptor 9 gene.},
  author={Omkar Byadgi and Dinda Puteri and Yan-Horn Lee and Jinwon Lee and Timothy Ghee Hock Cheng},
  journal={Fish & shellfish immunology},
  year={2014},
  volume={36 2},
  pages={
          417-27
        }
}
Cobia culture is hindered by bacterial infection (Photobacterium damselae subsp. piscicida) and in order to study the effect of P. damselae subsp. piscicida challenge and CpG ODN stimulation on cobia Toll like receptor 9 (RCTLR9), we used PCR to clone RCTLR9 gene and qRT-PCR to quantify gene expression. The results indicated that RCTLR9 cDNA contains 3141 bp. It encodes 1047 amino acids containing 16 typical structures of leucine-rich repeats (LRRs) including an LRRTYP, LRRCT and a motif… CONTINUE READING
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