Identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (Ursus arctos)

@article{Kleven2012IdentificationAE,
  title={Identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (Ursus arctos)},
  author={Oddmund Kleven and Bj{\"o}rn M. Hallstr{\"o}m and Frank Hailer and Axel Janke and Snorre B. Hagen and Alexander Kopatz and Hans Geir Eiken},
  journal={Conservation Genetics Resources},
  year={2012},
  volume={4},
  pages={737-741}
}
We here describe 34 novel microsatellite loci from the brown bear (Ursus arctos) identified using a next-generation sequencing approach. While we found allelic variation in 30 of these, we selected 16 polymorphic microsatellites (11 di- and 5 tetranucleotide repeats) for four multiplex-PCR assays based on genetic variability, PCR performance and interpretability. Applying these assays for the 16 novel microsatellites, we screened 31 brown bears from a northern European population. The number of… CONTINUE READING
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