Identification and dynamic modeling of biomarkers for bacterial uptake and effect of sulfonamide antimicrobials.

  title={Identification and dynamic modeling of biomarkers for bacterial uptake and effect of sulfonamide antimicrobials.},
  author={Merle K Richter and A. Focks and B. Siegfried and D. Rentsch and M. Krauss and R. Schwarzenbach and J. Hollender},
  journal={Environmental pollution},
The effects of sulfathiazole (STA) on Escherichia coli with glucose as a growth substrate was investigated to elucidate the effect-based reaction of sulfonamides in bacteria and to identify biomarkers for bacterial uptake and effect. The predominant metabolite was identified as pterine-sulfathiazole by LC-high resolution mass spectrometry. The formation of pterine-sulfathiazole per cell was constant and independent of the extracellular STA concentrations, as they exceeded the modeled half… Expand
Co-metabolism of sulfamethoxazole by a freshwater microalga Chlorella pyrenoidosa.
Microalgae-mediated biodegradation of antibiotics has recently gained increased attention from international scientific community. However, limited information is available regardingExpand
Biotransformation of Sulfonamide Antibiotics in Activated Sludge: The Formation of Pterin-Conjugates Leads to Sustained Risk.
It is shown that pterin-sulfonamide conjugates represent a major biotransformation route for sulfonamides in laboratory batch experiments with activated sludge and suggest that risk from exposure to sulfonamide antibiotics may be less reduced during wastewater treatment than previously assumed. Expand
Exploring micropollutant biotransformation in three freshwater phytoplankton species.
Phytoplankton constitute an important component of surface water ecosystems; however little is known about their contribution to biotransformation of organic micropollutants, including fungicides and 9 pharmaceuticals. Expand
On Biotransformation of Polar Organic Micropollutants in Phytoplankton
Online solid-phase extraction was combined with nano-liquid chromatography coupled to highresolution mass spectrometry (HRMS) for the analysis of micropollutants in environmental samples from smallExpand


Mechanistic link between uptake of sulfonamides and bacteriostatic effect: model development and application to experimental data from two soil microorganisms.
A mechanistic dose-response relationship is developed and evaluated against previously published data, where differences in the responses of Pseudomonas aeruginosa and Panthoea agglomerans toward changing medium pH values were found, most likely as a result of their diverse pH regulation. Expand
A mechanistical model for the uptake of sulfonamides by bacteria.
The pH-dependent intracellular accumulation of various sulfonamides correlates well with their observed antibiotic effect on selected bacteria and depends on the bacterial pH-regulation mechanisms. Expand
The characteristics and significance of sulfonamides as substrates for Escherichia coli dihydropteroate synthase.
It is shown that sulfanilamide, sulfathiazole, and sulfamethoxazole are efficient alternate substrates for this reaction, with apparent K,,, values equivalent to their Ki values as competitive inhibitors. Expand
Herbicidal effects of sulfamethoxazole in Lemna gibba: using p-aminobenzoic acid as a biomarker of effect.
Analysis of pABA concentrations appears to provide a sulfonamide-specific biomarker of effect based on MOA with exceptional diagnostic capacity and sensitivity compared to traditional morphological end points, suggesting that, as in bacteria, sulf onamide antibiotics specifically disrupt folate biosynthesis via inhibition of DHPS. Expand
Growth-inhibitory effects of sulfonamides at different pH: dissimilar susceptibility patterns of a soil bacterium and a test bacterium used for antibiotic assays.
The pH dependent mode of reaction of different bacteria to sulfonamides may additionally govern the population dynamics in soils and experimental and modeling results could be brought into good agreement for P. agglomerans if the cell-internal pH was admitted to approximate the external pH for modeling calculations. Expand
pH dependency in uptake of sulfonamides by bacteria.
Sulfonamide-sensitive cells of Escherichia coli were incubated under standarized conditions with various sulfonamides and the quantity of sulfonamide uptake into the bacteria measured and a pH dependency was detected which varied with the pKa values of the substances tested. Expand
Kinetics and mechanisms of action of drugs on microorganisms. VII. Quantitative adherence of sulfonamide action on microbial growth to a receptor-site model.
Variations of sulfonamide concentrations after the lag phase demonstrate that reversibility exists and the ultimate kapp. Expand
Effects of sulfamethoxazole on soil microbial communities after adding substrate.
Although different variables showed different sensitivities to the effects of SMX, the results were consistent with an initial decrease in bacterial growth rates of sensitive species, which eventually transformed into more tolerant species, altering the community composition. Expand
Cation binding of antimicrobial sulfathiazole to leonardite humic acid.
High affinity cation binding explains sorption of polar sulfonamides in agricultural soils and the strong dependence of sorption on SOM content and pH. Expand
Sulfonamides in the environment as veterinary drugs.
Preerential flow in clay soils has been identified as a mechanism responsible for surface water contamination by SAs. Expand