Identification and distribution of a novel, collagen-binding protein in the developing subepicardium and endomysium.

@article{Tidball1992IdentificationAD,
  title={Identification and distribution of a novel, collagen-binding protein in the developing subepicardium and endomysium.},
  author={James G. Tidball},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 29},
  pages={21211-9}
}
A protein doublet (M(r) = 68,000) that copurifies with chicken cardiac collagen types I and III is purified and characterized in the present study. Peptide mapping and amino terminus sequencing for both 68-kDa polypeptides show they have similar structures. This is supported by amino terminus sequencing of a 39-kDa proteolytic fragment of each polypeptide. The 68-kDa polypeptides appear at pI 6.7-6.8 in two-dimensional gels. Under nonreducing, electrophoretic conditions, the doublet appears as… CONTINUE READING