Identification and distribution of 5-HT3 receptors in rat brain using radioligand binding

  title={Identification and distribution of 5-HT3 receptors in rat brain using radioligand binding},
  author={Gavin Kilpatrick and Brian J. Jones and Michael Brian Tyers},
Functional serotonin (5-hydroxytryptamine, 5-HT) receptors have been divided into three subtypes: 5-HT1,-like, 5-HT2 and 5-HT3, (ref. 1). Brain binding sites have been identified for both the 5-HT1, and 5-HT2 subtypes. Receptors of the 5-HT3 type have been characterized on isolated peripheral tissue models such as the rat vagus nerve2, guinea-pig ileum3 and isolated rabbit heart4. Using these models, selective 5-HT3 receptor antagonists such as MDL 72222 (ref. 5), ICS 205-930 (ref. 6), GR38032F… Expand
Regional differences in the transduction mechanisms of 5-hydroxytryptamine receptors in the mammalian brain
It could be established that 5-HT1 sites are a mixture of 4 distinct classes of high affinity sites for [3H]5-HT, whose proportions are extremely variable from one brain area to another, and also from one species to another. Expand
Serotonin 5‐HT1D Receptors
5- HT1D receptors are negatively coupled to adenylate cyclase in guinea-pig and calf substantia nigra, and 5-HT1B receptors are positively coupled toAdenylated cyclaseIn rat substantia Nigra, which suggests that the canine basilar artery and saphenous vein, described as models for "5-HT 1-like" receptors, could contain 5- HT 1D receptors. Expand
Autoradiographic mapping of 5-HT1 receptors in the guinea-pig brain with particular reference to the 5-HT1D receptor sites
The present results indicate that the guinea-pig is a useful laboratory animal for the study of 5-HT1D receptors, and provides a detailed map of the 5- HT1 receptors in the Guinea-Pig brain. Expand
Differential Composition of 5-Hydroxytryptamine3Receptors Synthesized in the Rat CNS and Peripheral Nervous System
The in situ hybridization histochemistry and reverse transcriptase-PCR amplification was used to demonstrate that 5-HT3Asubunit transcripts are expressed in central and peripheral neurons, and it was determined that neurons of NG projecting to the nucleus tractus solitarium and those of dorsal root ganglia projecting to superficial layers of the spinal cord express 5- HT3A/3B subunits. Expand
Anxiogenic and Other Effects of mCPP, a 5-HT, 1C Agonist
A decade ago, central 5-HT receptors were classified into 5-HT1 and 5-HT2 types (Peroutka and Snyder, 1979). More recently, 5-HT3 receptors were recognized (Kilpatrick et al., 1987) and 5-HT4 sitesExpand
Alterations in serotonin binding sites after 5,7-dihydroxytryptamine treatment in the rat spinal cord
It appears that an up-regulation of 5-HT1B sites occurred that may have obscured the ability to observe a reduction in a small population of sites located on the serotonergic nerve terminals. Expand
Multiple Serotonin Receptors in the Human Brain
The presence of very high densities of 5-HT1C sites over the choroid plexus suggest a role for this receptor in regulating the production and composition of the cerebrospinal fluid, and the quantitative autoradiographical analysis allows the identification of microscopic brain areas, which are highly enriched in subtypes of5-HT receptor. Expand
Stereological investigation of 5-HT3 receptors in the substantia nigra and dorsal raphe nucleus in the rat
The results support the presence of 5-HT3 receptors in the SN, but not in the DRN, and do not support their expression on monoaminergic cells within these two brain areas. Expand
5-HT1D receptors in guinea-pig and pigeon brain
It is suggested that 5-HT1D sites may be present in the brain of the majority of vertebrate species located higher than the sauropside-mammalian divergence in the phylogenic tree, whereas 5- HT1B sites are only found in some rodents. Expand