Identification and cyclic AMP-induced modification of the cyclic AMP receptor in Dictyostelium discoideum.

@article{Klein1985IdentificationAC,
  title={Identification and cyclic AMP-induced modification of the cyclic AMP receptor in Dictyostelium discoideum.},
  author={Peter W. Klein and Anne Theibert and D L Fontana and Peter N. Devreotes},
  journal={The Journal of biological chemistry},
  year={1985},
  volume={260 3},
  pages={1757-64}
}
We have recently identified a cell surface cAMP-binding protein by specific photoaffinity labeling of intact Dictyostelium discoideum cells with 8-N3-[32P] cAMP. The major photolabeled protein appears as a doublet (Mr = 40,000-43,000) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis autoradiography. In this study, the doublet is shown to have the characteristics of the cAMP receptor responsible for chemotaxis and cAMP signaling. Both specific photoaffinity labeling of the doublet… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 19 extracted citations

References

Publications referenced by this paper.

Similar Papers

Loading similar papers…