Identification and comparison of CD34-positive cells and their subpopulations from normal peripheral blood and bone marrow using multicolor flow cytometry.

@article{Bender1991IdentificationAC,
  title={Identification and comparison of CD34-positive cells and their subpopulations from normal peripheral blood and bone marrow using multicolor flow cytometry.},
  author={James G. Bender and K L Unverzagt and Don E. Walker and W Lee and Dennis E. Van Epps and Douglas H. Smith and Carleton C. Stewart and Luen Bik To},
  journal={Blood},
  year={1991},
  volume={77 12},
  pages={2591-6}
}
Four-color flow cytometry was used with a cocktail of antibodies to identify and isolate CD34+ hematopoietic progenitors from normal human peripheral blood (PB) and bone marrow (BM). Mature cells that did not contain colony forming cells were resolved from immature cells using antibodies for T lymphocytes (CD3), B lymphocytes (CD20), monocytes (CD14), and granulocytes (CD11b). Immature cells were subdivided based on the expression of antigens found on hematopoietic progenitors (CD34, HLA-DR… CONTINUE READING

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