Identification and characterization of thymosin beta-4 in chicken macrophages using whole cell MALDI-TOF.

@article{Kannan2007IdentificationAC,
  title={Identification and characterization of thymosin beta-4 in chicken macrophages using whole cell MALDI-TOF.},
  author={Lakshmi Kannan and Narayan C. Rath and Rohana Liyanage and Jackson O. Lay},
  journal={Annals of the New York Academy of Sciences},
  year={2007},
  volume={1112},
  pages={425-34}
}
The aim of the study was to determine chicken monocyte- and granulocyte-associated peptides and proteins using "whole cell" matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and to characterize the peptides based on their abundance. The mass spectra showed a prominent peak at m/z 4963 in monocytes/macrophages but not in the granulocytes. Subsequent purification and characterization of the m/z 4963 peptide from an avian macrophage cell line HTC, revealed… CONTINUE READING

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