Identification and characterization of the genes encoding three structural proteins of the Sulfolobus virus-like particle SSV1

@article{Reiter2004IdentificationAC,
  title={Identification and characterization of the genes encoding three structural proteins of the Sulfolobus virus-like particle SSV1},
  author={W. Reiter and P. Palm and A. Henschen and F. Lottspeich and W. Zillig and B. Grampp},
  journal={Molecular and General Genetics MGG},
  year={2004},
  volume={206},
  pages={144-153}
}
SummaryThree structural proteins, VP1, VP2 and VP3, of the virus-like particle SSV1 of the thermoacidophilic archaebacterium Sulfolobus sp. strain B12 were purified. VP1 and VP3 are very hydrophobic and show a high degree of homology. They consist of 73 and 92 amino acid residues, respectively. The third protein, VP2, is extremely basic containing 29 basic amino acids but only 4 acidic ones in a total of 74 amino acid residues. The genes encoding these three proteins were mapped within the… Expand

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References

SHOWING 1-10 OF 60 REFERENCES
Structure and sequence divergence of two archaebacterial genes.
TLDR
Based on the degree of hisA sequence homology, it is concluded that M. voltae and M. vannielii are less closely related taxonomically than are members of the enteric group of eubacteria. Expand
Structure of methanogen genes
TLDR
An analysis of the structure and presumed regulatory signals in sequenced methanogen genes is presented and it is indicated that a consensus core ribosome binding sequence for methanogens would be 5 'AGGTGA and that many of the ribosom binding sequences are, in fact, longer than the core sequence containing 7 bases with the potential to hybridize to 16SrRNA. Expand
Structure of genes and an insertion element in the methane producing archaebacterium Methanobrevibacter smithii
TLDR
Sequence analyses, transposon mutagenesis and expression in E. coli minicells indicate that purE and proC complementations result from the synthesis of M. smithii polypeptides, and comparison of data with sequences from halophilic archaebacteria suggests that 5′GAANTTTCA and 5′TTTTAATATAAA may be consensus promoter sequences for archaEBacteria. Expand
Physical mapping of genes coding for two subunits of methyl CoM reductase component C of Methanococcus voltae
SummaryA genomic library of EcoRI*-digested cellular DNA from Methanococcus voltae was constructed in an expression vector, which allows the induction of fusion proteins of the Mc. voltae geneExpand
Archaebacterial phylogeny: perspectives on the urkingdoms.
TLDR
By 16S rRNA sequence measure the archaebacteria constitute a phylogenetically coherent grouping (clade), which excludes both the eubacteria and the eukaryotes--a conclusion that is supported by other sequence evidence as well. Expand
TTV1, TTV2 and TTV3, a family of viruses of the extremely thermophilic, anaerobic, sulfur reducing archaebacterium Thermoproteus tenax
TLDR
Three different temperent viruses of the extremely thermophilic chemolithoautotrophic archaebacterium, Thermoproteus tenax, TTV1,TTV2 and TTV3, each contain linear, double-stranded DNA and have neither homologies with each other nor with the host. Expand
Structural homology between different archaebacterial DNA‐dependent RNA polymerases analyzed by immunological comparison of their components
The archaebacterial DNA‐dependent RNA polymerases have a complex structure containing eight or more components. Immunochemical analysis shows an extensive homology between the components of theExpand
Sequence of the 16S rRNA gene from the thermoacidophilic archaebacteriumSulfolobus solfataricus and its evolutionary implications
TLDR
The S.S. solfataricus sequence is specifically related to those of the other archaebacteria, to the exclusion of the eukaryotic and eubacterial sequences, when examined either by evolutionary distance matrix analyses or by the criterion of minimum change (maximum parsimony). Expand
Sequence analysis of the insertion element ISH1.8 and of associated structural changes in the genome of phage ΦH of the archaebacterium Halobacterium halobium
TLDR
The insertion element ISH1.8 is sequenced which can be present in one or two copies in the genome of phage ΦH of Halobacterium halobium and is released from the phage genome by recombination within a direct repeat of 9 bp. Expand
The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
  • J. Shine, L. Dalgarno
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1974
TLDR
It is suggested that this region of the RNA is able to interact with mRNA and that the 3'-terminal U-U-A(OH) is involved in the termination of protein synthesis through base-pairing with terminator codons. Expand
...
1
2
3
4
5
...