Identification and characterization of six new alternatively spliced variants of the human μ opioid receptor gene, Oprm

@article{Pan2005IdentificationAC,
  title={Identification and characterization of six new alternatively spliced variants of the human $\mu$ opioid receptor gene, Oprm
},
  author={Ling Pan and J. -Y. Xu and Rui Yu and M.-M. Xu and Y.-X. Pan and Gavril W. Pasternak},
  journal={Neuroscience},
  year={2005},
  volume={133},
  pages={209-220}
}
The mu opioid receptor plays an important role in mediating the actions of morphine and morphine-like drugs. Receptor binding and a wide range of pharmacological studies have proposed several mu receptor subtypes, but only one mu opioid receptor (Oprm) gene has been isolated. Like the mouse and rat, the human Oprm gene undergoes alternative splicing. In the present studies, we have identified and characterized six new splice variants from the human Oprm gene using a reverse transcription… Expand
Diversity and complexity of the mu opioid receptor gene: alternative pre-mRNA splicing and promoters.
TLDR
Diversity and complexity of the Oprm gene was further demonstrated by functional differences in agonist-induced G protein activation, adenylyl cyclase activity, and receptor internalization among carboxyl terminal variants. Expand
Identification and characterization of seven new exon 11-associated splice variants of the rat mu opioid receptor gene, OPRM1
TLDR
The identification of the rat exon 11 and its associated variants further demonstrated conservation of 5' splicing in OPRM1 genes among rodents and humans. Expand
The opioid ligand binding of human mu-opioid receptor is modulated by novel splice variants of the receptor.
  • H. Choi, C. Kim, +6 authors H. Loh
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  • Biochemical and biophysical research communications
  • 2006
TLDR
Data support a potential role of SV1 and SV2 proteins as possible biological modulator of human mu-opioid receptor. Expand
Alternative Pre-mRNA Splicing of the Mu Opioid Receptor Gene, OPRM1: Insight into Complex Mu Opioid Actions
  • Shan Liu, Wen-Jia Kang, +6 authors Ying-Xian Pan
  • Medicine
  • Biomolecules
  • 2021
TLDR
The OPRM1 variants can be targeted for development of novel opioid analgesics that are potent against multiple types of pain, but devoid of many side-effects associated with traditional opiates. Expand
Isolation and characterization of new exon 11‐associated N‐terminal splice variants of the human mu opioid receptor gene
TLDR
The presence of exon 11‐associated variants in humans raises questions regarding their potential role in heroin and morphine‐6β‐glucuronide actions in people as they do in mice, and region‐specific alternative splicing is suggested. Expand
Identification of Four Novel Exon 5 Splice Variants of the Mouse μ-Opioid Receptor Gene: Functional Consequences of C-Terminal Splicing
TLDR
In conclusion, alterations in the amino acid sequence of the C terminus do not alter the μ-specificity of the receptor but they can influence the binding characteristics, efficacy, and potency of μ-opioids. Expand
Endogenous Opioid Peptides and Alternatively Spliced Mu Opioid Receptor Seven Transmembrane Carboxyl-Terminal Variants
TLDR
The results from early pharmacological studies, including receptor binding affinity and G protein activation, and recent studies of β-arrestin2 recruitment and biased signaling are summarized to provide new insights into the mechanisms and functions of endogenous opioid peptides, which are mediated through the OPRM1 7TM C-terminal splice variants. Expand
Molecular assays for characterization of alternatively spliced isoforms of the u opioid receptor (MOR).
TLDR
The methodologies used to assay key mediators of MOR activation including cellular assays for cAMP, free Ca(2+), and NO, all of which have been implicated in the pharmacological effects of MOR agonists are described. Expand
Molecular Biology of Mu Opioid Receptors
TLDR
Both sets of variants are functionally important, as shown by the actions of opioids in their respective knockout mice, providing a new perspective on understanding complex Mu opioid actions in animals and humans. Expand
Modulation of OPRM1 Alternative Splicing by Morphine and HIV-1 Nef.
TLDR
HIV-1 may alter MOR isoform expression with Nef protein by amplifying the rate of MOR-1X alternative splicing induced by morphine, which is suggested to alter OPRM1 pre-mRNA splicing. Expand
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