Identification and characterization of side population cells in embryonic stem cell cultures.

@article{Vieyra2009IdentificationAC,
  title={Identification and characterization of side population cells in embryonic stem cell cultures.},
  author={Diego S. Vieyra and Allison Rosen and Margaret A. Goodell},
  journal={Stem cells and development},
  year={2009},
  volume={18 8},
  pages={1155-66}
}
Marker and functional heterogeneity has been described for embryonic stem cells (ESCs). This property has been correlated with the presence of ESC subpopulations resembling pluripotent cell lineages of the embryo. The ability to efflux Hoechst (Ho) displayed by side population (SP) cells has proven valuable as a marker to identify multipotent stem cells from a variety of tissues. Here we report that cultures from different ESC lines consistently show an SP population that displays antigens of… CONTINUE READING

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