Identification and characterization of phosphorylated proteins in the human pituitary

@article{Giorgianni2004IdentificationAC,
  title={Identification and characterization of phosphorylated proteins in the human pituitary},
  author={Francesco Giorgianni and Sarka Beranova‐Giorgianni and Dominic M. Desiderio},
  journal={PROTEOMICS},
  year={2004},
  volume={4}
}
Post‐translational modifications of proteins from the human pituitary gland play an important role in the regulation of different body functions. We report on the application of a liquid chromatography‐tandem mass spectrometry (MS/MS) based approach to detect and characterize phosphorylated proteins in a whole human pituitary digest. By combining an immobilized metal affinity column‐based enrichment method with MS/MS conditions that favor the neutral loss of phosphoric acid from a… 
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