Identification and characterization of protein interactions in the mammalian mRNA processing body using a novel two-hybrid assay.

@article{Bloch2011IdentificationAC,
  title={Identification and characterization of protein interactions in the mammalian mRNA processing body using a novel two-hybrid assay.},
  author={Donald B Bloch and Rita A Nobre and Gillian A Bernstein and Wei-hong Yang},
  journal={Experimental cell research},
  year={2011},
  volume={317 15},
  pages={2183-99}
}
Components of the mRNA processing body (P-body) regulate critical steps in mRNA storage, transport, translation and degradation. At the core of the P-body is the decapping complex, which removes the 5' cap from de-adenylated mRNAs and mediates an irreversible step in mRNA degradation. The assembly of P-bodies in Saccharomyces cerevisiae, Arabidopsis thaliana and Drosophila melanogaster has been previously described. Less is known about the assembly of mammalian P-bodies. To investigate the… CONTINUE READING