Identification and characterization of novel esterases from a deep-sea sediment metagenome

  title={Identification and characterization of novel esterases from a deep-sea sediment metagenome},
  author={Xiawei Jiang and Xuewei Xu and Yingyi Huo and Yuehong Wu and Xufen Zhu and Xinqi Zhang and Min Wu},
  journal={Archives of Microbiology},
  • Xiawei Jiang, Xuewei Xu, +4 authors Min Wu
  • Published 2011
  • Biology, Medicine
  • Archives of Microbiology
  • A deep-sea sediment metagenomic library was constructed and screened for lipolytic enzymes by activity-based approach. Nine novel lipolytic enzymes were identified, and the amino acid sequences shared 56% to 84% identity to other lipolytic enzymes in the database. Phylogenetic analysis showed that these enzymes belonged to family IV lipolytic enzymes. One of the lipolytic enzymes, Est6, was successfully cloned and expressed in Escherichia coli Rosetta in a soluble form. The recombinant protein… CONTINUE READING
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