Identification and characterization of novel cellulolytic and hemicellulolytic genes and enzymes derived from German grassland soil metagenomes

@inproceedings{Nacke2011IdentificationAC,
  title={Identification and characterization of novel cellulolytic and hemicellulolytic genes and enzymes derived from German grassland soil metagenomes},
  author={Heiko Nacke and Martin Engelhaupt and Silja Brady and Christiane Fischer and Janine Tautzt and Rolf Daniel},
  booktitle={Biotechnology Letters},
  year={2011}
}
Soil metagenomes represent an unlimited resource for the discovery of novel biocatalysts from soil microorganisms. Three large-inserts metagenomic DNA libraries were constructed from different grassland soil samples and screened for genes conferring cellulase or xylanase activity. Function-driven screening identified a novel cellulase-encoding gene (cel01) and two xylanase-encoding genes (xyn01 and xyn02). From sequence and protein domain analyses, Cel01 (831 amino acids) belongs to glycoside… CONTINUE READING