Identification and characterization of native proteins of Escherichia coli BL-21 that display affinity towards Immobilized Metal Affinity Chromatography and Hydrophobic Interaction Chromatography Matrices.

@article{Tiwari2010IdentificationAC,
  title={Identification and characterization of native proteins of Escherichia coli BL-21 that display affinity towards Immobilized Metal Affinity Chromatography and Hydrophobic Interaction Chromatography Matrices.},
  author={Neha Tiwari and Lauren Woods and Ryan Haley and Alicia D Kight and Robyn L. Goforth and Kevin P Clark and Mohammad M. Ataai and Ralph L Henry and Robert R. Beitle},
  journal={Protein expression and purification},
  year={2010},
  volume={70 2},
  pages={191-5}
}
The purpose of this study was to identify and characterize Escherichia coli proteins which display affinity towards both Immobilized Metal Affinity Chromatography (IMAC) and Hydrophobic Interaction Chromatography (HIC). Co(II) IMAC was chosen as the primary capture step, followed by HIC employing different concentrations of salt to promote adsorption… CONTINUE READING