Identification and characterization of podocalyxin--the major sialoprotein of the renal glomerular epithelial cell

@article{Kerjaschki1984IdentificationAC,
  title={Identification and characterization of podocalyxin--the major sialoprotein of the renal glomerular epithelial cell},
  author={Dontscho Kerjaschki and David J Sharkey and Marilyn Gist Farquhar},
  journal={The Journal of Cell Biology},
  year={1984},
  volume={98},
  pages={1591 - 1596}
}
The glomerular epithelial polyanion is a specialized cell surface component found on renal glomerular epithelial cells (podocytes) that is rich in sialoprotein(s), as detected by staining with cationic dyes (colloidal iron, alcian blue) and wheat germ agglutinin (WGA). We have isolated rat glomeruli and analyzed their protein composition by SDS PAGE in 5-10% gradient gels. When the gels were stained with alcian blue or "Stains All," a single band with an apparent Mr of 140,000 was detected that… CONTINUE READING