Identification and characterization of gshA, a gene encoding the glutamate-cysteine ligase in the halophilic archaeon Haloferax volcanii.

@article{Malki2009IdentificationAC,
  title={Identification and characterization of gshA, a gene encoding the glutamate-cysteine ligase in the halophilic archaeon Haloferax volcanii.},
  author={Liron Malki and Michaela Yanku and Ilya Borovok and Gerald Cohen and Moshe Mevarech and Yair Aharonowitz},
  journal={Journal of bacteriology},
  year={2009},
  volume={191 16},
  pages={5196-204}
}
Halophilic archaea were found to contain in their cytoplasm millimolar concentrations of gamma-glutamylcysteine (gamma GC) instead of glutathione. Previous analysis of the genome sequence of the archaeon Halobacterium sp. strain NRC-1 has indicated the presence of a sequence homologous to sequences known to encode the glutamate-cysteine ligase GshA. We report here the identification of the gshA gene in the extremely halophilic archaeon Haloferax volcanii and show that H. volcanii gshA directs… CONTINUE READING