Identification and characterization of biomarkers of organophosphorus exposures in humans.

@article{Kim2010IdentificationAC,
  title={Identification and characterization of biomarkers of organophosphorus exposures in humans.},
  author={Jerry H. Kim and Richard C. Stevens and Michael J. MacCoss and David R. Goodlett and Alexander Scherl and Rebecca J. Richter and Stephanie M. Suzuki and Clement E. Furlong},
  journal={Advances in experimental medicine and biology},
  year={2010},
  volume={660},
  pages={
          61-71
        }
}
Over 1 billion pounds of organophosphorus (OP) chemicals are manufactured worldwide each year, including 70 million pounds of pesticides sprayed in the US. Current methods to monitor environmental and occupational exposures to OPs such as chlorpyrifos (CPS) have limitations, including low specificity and sensitivity, and short time windows for detection. Biomarkers for the OP tricresyl phosphate (TCP), which can contaminate bleed air from jet engines and cause an occupational exposure of… 
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References

SHOWING 1-10 OF 69 REFERENCES
Biomarkers of organophosphorus (OP) exposures in humans.
Mass Spectrometric Analyses of Organophosphate Insecticide Oxon Protein Adducts
TLDR
MS is a useful and emerging tool for the identification of proteins that are modified by activated organophosphate insecticides and can characterize the structure of the OP adduct and also the specific amino acid residue that forms the key bond with the OP.
Albumin, a new biomarker of organophosphorus toxicant exposure, identified by mass spectrometry.
TLDR
It is concluded that OP bound to albumin could serve as a new biomarker of OP exposure in man because humans have no carboxylesterase in blood.
LC-MS-based procedures for monitoring of toxic organophosphorus compounds and verification of pesticide and nerve agent poisoning
TLDR
LC-MS-MS detection of phosphylated peptides generated from inhibited circulating serum butyrylcholinesterase by valuable proteomics techniques enables proof of intoxication on the molecular level, and a comprehensive overview on the status quo of LC- MS-based OPC analysis is given.
New method for retrospective detection of exposure to organophosphorus anticholinesterases: application to alleged sarin victims of Japanese terrorists.
TLDR
The new procedure is applied to serum samples from victims of the Tokyo subway attack by the AUM Shinriyko sect and from an earlier incident at Matsumoto, finding that these victims had been exposed to an organophosphate with the structure PriO(CH3)P(O)X, presumably with X = F (sarin).
Development of diagnostics in the search for an explanation of aerotoxic syndrome.
Blood acylpeptide hydrolase activity is a sensitive marker for exposure to some organophosphate toxicants.
TLDR
Blood APH activity is a sensitive marker for exposure to some but not all OP pesticides and chemical warfare agents and is emphasized as a potential marker of OP exposure.
Metabolism of chlorpyrifos by human cytochrome P450 isoforms and human, mouse, and rat liver microsomes.
  • J. Tang, Y. Cao, E. Hodgson
  • Biology, Chemistry
    Drug metabolism and disposition: the biological fate of chemicals
  • 2001
TLDR
Differences between individuals suggest that differential sensitivities to CPS may exist in the human population, and predictions of metabolic activation and/or detoxication could be made based on relative amounts of CYP2B6, 2C19, and 3A4 in the microsomes.
Identification of acylpeptide hydrolase as a sensitive site for reaction with organophosphorus compounds and a potential target for cognitive enhancing drugs.
TLDR
It was possible to show that acylpeptide hydrolase is the only enzyme in the brain capable of hydrolyzing the substrate N-acetyl-alanyl-p-nitroanilide, and suggest that it may be the target for the cognitive-enhancing effects of certain organophosphorus compounds.
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