Identification and characterization of acidic hydrolases with chitinase and chitosanase activities from sweet orange callus tissue.

@article{Osswald1994IdentificationAC,
  title={Identification and characterization of acidic hydrolases with chitinase and chitosanase activities from sweet orange callus tissue.},
  author={Wolfgang F. Osswald and Jeffrey P. Shapiro and Hamed Doostdar and Roy E. McDonald and Randall P. Niedz and Campbell J. Nairn and Clifford J. Hearn and Richard T. Mayer},
  journal={Plant & cell physiology},
  year={1994},
  volume={35 5},
  pages={811-20}
}
Acidic chitinases (EC 3.2.1.14) were isolated and characterized from 4-week-old nonembryogenic Citrus sinensis L. Osbeck cv 'Valencia' callus tissue. The enzymes were purified using size exclusion, anion exchange, and chromatofocusing HPLC techniques. Eleven isoforms were isolated with M(r)s between 26,000 and 37,400. Eight of the isoforms were purified to homogeneity, and all but one cross-reacted with a polyclonal antibody raised against a basic class I potato leaf chitinase. The isoelectric… CONTINUE READING