Identification and characterization of a well-defined series of coronatine biosynthetic mutants of Pseudomonas syringae pv. tomato DC3000.

@article{Brooks2004IdentificationAC,
  title={Identification and characterization of a well-defined series of coronatine biosynthetic mutants of Pseudomonas syringae pv. tomato DC3000.},
  author={David M Brooks and Gustavo Hern{\'a}ndez-Guzm{\'a}n and Andrew P. Kloek and Francisco J Alarcon-Chaidez and Aswathy Sreedharan and Vidhya Rangaswamy and Alejandro Pe{\~n}aloza-V{\'a}zquez and Carol L. Bender and Barbara N Kunkel},
  journal={Molecular plant-microbe interactions : MPMI},
  year={2004},
  volume={17 2},
  pages={162-74}
}
To identify Pseudomonas syringae pv. tomato genes involved in pathogenesis, we carried out a screen for Tn5 mutants of P. syringae pv. tomato DC3000 with reduced virulence on Arabidopsis thaliana. Several mutants defining both known and novel virulence loci were identified. Six mutants contained insertions in biosynthetic genes for the phytotoxin coronatine (COR). The P. syringae pv. tomato DC3000 COR genes are chromosomally encoded and are arranged in two separate clusters, which encode… CONTINUE READING
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