Identification and characterization of a biomineralization related gene PFMG1 highly expressed in the mantle of Pinctada fucata.

@article{Liu2007IdentificationAC,
  title={Identification and characterization of a biomineralization related gene PFMG1 highly expressed in the mantle of Pinctada fucata.},
  author={Hai-luo Liu and Shang-feng Liu and Ye-Jing Ge and Jing Liu and Xiaoyan Wang and Li-Ping Xie and Rong-qing Zhang and Zhao Wang},
  journal={Biochemistry},
  year={2007},
  volume={46 3},
  pages={844-51}
}
To elucidate the mechanism of nacre biomineralization, the mantle of Pinctada fucata (P. fucata) from the South China Sea was used. Using the mantle cDNA library and the ESTs we have cloned through suppression subtractive hybridization (SSH), ten novel genes including PFMG1 were obtained through nested PCR. Bioinformative results showed that PFMG1 had a high homology (40%) with Onchocerca volvulus calcium-binding protein CBP-1 and had two EF-hand calcium-binding domains from the 81st to the… CONTINUE READING