Identification and characterization of a novel biotin biosynthesis gene in Saccharomyces cerevisiae.

@article{Wu2005IdentificationAC,
  title={Identification and characterization of a novel biotin biosynthesis gene in Saccharomyces cerevisiae.},
  author={Hong Wu and Kiyoshi Ito and Hitoshi Shimoi},
  journal={Applied and environmental microbiology},
  year={2005},
  volume={71 11},
  pages={6845-55}
}
Yeast Saccharomyces cerevisiae cells generally cannot synthesize biotin, a vitamin required for many carboxylation reactions. Although sake yeasts, which are used for Japanese sake brewing, are classified as S. cerevisiae, they do not require biotin for their growth. In this study, we identified a novel open reading frame (ORF) in the genome of one strain of sake yeast that we speculated to be involved in biotin synthesis. Homologs of this gene are widely distributed in the genomes of sake… CONTINUE READING
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Methods in yeast genetics

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