Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase.

  title={Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase.},
  author={Pia Ek and Gunilla Pettersson and Bo Ek and Feng Gong and Jin-ping Li and Orjan Zetterqvist},
  journal={European journal of biochemistry},
  volume={269 20},
Protein histidine phosphorylation in eukaryotes has been sparsely studied compared to protein serine/threonine and tyrosine phosphorylation. In an attempt to rectify this by probing porcine liver cytosol with the phosphohistidine-containing peptide succinyl-Ala-His(P)-Pro-Phe-p-nitroanilide (phosphopeptide I), we observed a phosphatase activity that was insensitive towards okadaic acid and EDTA. This suggested the existence of a phosphohistidine phosphatase different from protein phosphatase 1… 

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