Identification and characterization of T cell-stimulating antigens from Leishmania by CD4 T cell expression cloning.

@article{Probst2001IdentificationAC,
  title={Identification and characterization of T cell-stimulating antigens from Leishmania by CD4 T cell expression cloning.},
  author={Peter Probst and Erika Stromberg and Hashim Warsama Ghalib and Michelle R Mozel and Roberto Badar{\'o} and Steven G Reed and John R. Webb},
  journal={Journal of immunology},
  year={2001},
  volume={166 1},
  pages={498-505}
}
Persistent immunity against Leishmania: infections in humans is mediated predominantly by CD4(+) T cells of the Th1 phenotype. Herein we report the expression cloning of eight Leishmania: Ags using parasite-specific T cell lines derived from an immune donor. The Ags identified by this technique include the flagellar proteins alpha- and beta-tubulin, histone H2b, ribosomal protein S4, malate dehydrogenase, and elongation factor 2, as well as two novel parasite proteins. None of these proteins… CONTINUE READING