Identification and characterization of E. coli CRISPR‐cas promoters and their silencing by H‐NS

@article{Pul2010IdentificationAC,
  title={Identification and characterization of E. coli CRISPR‐cas promoters and their silencing by H‐NS},
  author={{\"U}mit Pul and Reinhild Wurm and Zihni Arslan and Ren{\'e} Geissen and Nina Hofmann and Rolf Wagner},
  journal={Molecular Microbiology},
  year={2010},
  volume={75}
}
Inheritable bacterial defence systems against phage infection and foreign DNA, termed CRISPR (clustered regularly interspaced short palindromic repeats), consist of cas protein genes and repeat arrays interspaced with sequences originating from invaders. The Cas proteins together with processed small spacer‐repeat transcripts (crRNAs) cause degradation of penetrated foreign DNA by unknown mechanisms. Here, we have characterized previously unidentified promoters of the Escherichia coli CRISPR… 
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258 Citations
Highly Influential Citations
24
Background Citations
103
Methods Citations
12
Results Citations
2

258 Citations

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Citation Type

Envelope stress is a trigger of CRISPR RNA‐mediated DNA silencing in Escherichia coli
TLDR
It is confirmed that the CasCDE enzymes form a stable ternary complex and appear to function as the catalytic core of the Cas system to process CRISPR RNA into its mature form.
Functional Characterization of CRISPR-Cas System in the Ethanologenic Bacterium Zymomonas mobilis ZM4
TLDR
Conclusively, the Type I-F CRISPR-Cas system in Z. mobilis ZM4 is active to functionally defense the invading DNA elements.
H‐NS‐mediated repression of CRISPR‐based immunity in Escherichia coli K12 can be relieved by the transcription activator LeuO
TLDR
It is demonstrated that in E. coli H‐NS and LeuO are antagonistic regulators of CRISPR‐based immunity, which leads to enhanced protection against phage infection.
CRISPR: A Bacterial Immunity System Based on Small RNAs
TLDR
The CRISPR system has been proven to confer adaptive and inheritable immunity against foreign nucleic acid and resembles the RNA interference phenomenon of eukaryotes, an important control and defense tool with widespread applications in research and medical developments.
Correction: CRISPR Content Correlates with the Pathogenic Potential of Escherichia coli
TLDR
Findings support an evolutionary connection between CRISPR and pathogenicity in E. coli.
Modulation of CRISPR locus transcription by the repeat-binding protein Cbp1 in Sulfolobus
TLDR
It is proposed that Cbp1 modulates production of longer pre-crRNA transcripts from CRISPR loci, a possible mechanism is that it minimizes interference from potential transcriptional signals carried on spacers deriving from A-T-rich genetic elements and, occasionally, on DNA repeats.
CRISPR Content Correlates with the Pathogenic Potential of Escherichia coli
TLDR
Findings support an evolutionary connection between CRISPR and pathogenicity in E. coli.
StpA represses CRISPR-Cas immunity in H-NS deficient Escherichia coli.
Transcription, processing and function of CRISPR cassettes in Escherichia coli
TLDR
Using an engineered strain with genomically located spacer matching phage λ, it is shown that endogenous levels of CRISPR cassette and cas genes expression allow only weak protection against infection with the phage, however, derepression of the CRISpr/Cas system by disruption of the hns gene leads to high level of protection.
Mechanism and engineering of CRISPR-associated endonucleases
TLDR
It is revealed that Cas9 can be programmed to target single-stranded RNA substrates for both high-affinity binding and site- specific cleavage using PAM-presenting oligonucleotides and that RNA targeting by Cas9 has the potential to transform the study of RNA function, much as site-specific DNA targeting has revolutionized genetic and genomic research.
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References

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