Identification and characterization of CAMP cohemolysin as a potential virulence factor of Riemerella anatipestifer.

@article{Crasta2002IdentificationAC,
  title={Identification and characterization of CAMP cohemolysin as a potential virulence factor of Riemerella anatipestifer.},
  author={Karen C Crasta and K Chua and Sumathi Subramaniam and Joachim Frey and Hilda Loh and H Tan},
  journal={Journal of bacteriology},
  year={2002},
  volume={184 7},
  pages={1932-9}
}
Riemerella anatipestifer is responsible for exudative septicemia in ducks. The genetic determinant of the CAMP cohemolysin, cam, from a strain of R. anatipestifer was cloned and expressed in Escherichia coli. Chromosomal DNA from serotype 19 strain 30/90 was used to construct a gene library in pBluescript II SK(-) vector in E. coli XL-1-Blue strain. The clones containing recombinant plasmids were screened for the CAMP reaction with Staphylococcus aureus. Those that showed cohemolysis were… CONTINUE READING

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