Identification and characterisation of the excreted/secreted serine proteases of larvae of the old world screwworm fly, Chrysomya bezziana.

@article{Muharsini2000IdentificationAC,
  title={Identification and characterisation of the excreted/secreted serine proteases of larvae of the old world screwworm fly, Chrysomya bezziana.},
  author={S Muharsini and Sukarsih and George Riding and S. Partoutomo and Stephen Hamilton and Peter Willadsen and Gene Wijffels},
  journal={International journal for parasitology},
  year={2000},
  volume={30 6},
  pages={705-14}
}
Serine proteases are the major proteolytic activity excreted or secreted from Chrysomya bezziana larvae as demonstrated by gelatin gel analyses and the use of specific substrates, benzoyl-Arg-p-nitroanilide and succinyl-Ala-Ala-Pro-Phe-p-nitroanilide. Serine proteases were identified through their inhibition by 4-(2-aminoethyl)-benzene sulphonyl fluoride and classified as trypsin- and chymotrypsin-like on the basis of inhibition by tosyl-L-lysine chloromethyl ketone and tosyl-L-phenylalanine… CONTINUE READING