Identification and characterisation of novel Pichia pastoris promoters for heterologous protein production.

@article{Stadlmayr2010IdentificationAC,
  title={Identification and characterisation of novel Pichia pastoris promoters for heterologous protein production.},
  author={Gerhard Stadlmayr and Astrid Mecklenbr{\"a}uker and Marion Rothm{\"u}ller and Michael Maurer and Michael G Sauer and Diethard Mattanovich and Brigitte Gasser},
  journal={Journal of biotechnology},
  year={2010},
  volume={150 4},
  pages={519-29}
}
The number of available promoters for the protein production host Pichia pastoris is limited, and in most applications comprises the methanol inducible alcohol oxidase 1 (AOX1) promoter and the constitutive glyceraldehyde phosphate dehydrogenase (GAP) promoter. To close this gap, we identified 24 novel potential regulatory sequences and tested their applicability to drive the expression of both intracellular as well as secretory heterologous proteins. While more than 80% of the promoters… CONTINUE READING

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