Identification and biochemical analysis of GRIN1 and GRIN2.

@article{Iida2004IdentificationAB,
  title={Identification and biochemical analysis of GRIN1 and GRIN2.},
  author={Naoyuki Iida and Tohru Kozasa},
  journal={Methods in enzymology},
  year={2004},
  volume={390},
  pages={
          475-83
        }
}
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References

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A Candidate Target for G Protein Action in Brain*
TLDR
Coexpression of GRIN1 or GRIN2 with activated Goα causes formation of a network of fine processes in Neuro2a cells, suggesting that these pathways may function downstream of Goα to control growth of neurites.
The α Subunits of Gz and Gi Interact with the eyes absent Transcription Cofactor Eya2, Preventing Its Interaction with the Six Class of Homeodomain-containing Proteins*
TLDR
A novel functional regulation of a co-activator directly by G protein subunits is shown, which is normally achieved indirectly via one or more intermediates.
Activation of the ERK/MAPK pathway by an isoform of rap1GAP associated with Gαi
TLDR
It is shown that a novel isoform of Rap1 GTPase-activating protein, called rap1GAPII, binds specifically to the α-subunits of the Gi family of heterotrimeric G-proteins, which activates the Ras-ERK/MAPK mitogenic pathway by membrane recruitment of rap1 GAPII and reduction of GTP-bound Rap1.
Protein Kinase C Phosphorylates G12α and Inhibits Its Interaction with Gβγ(*)
TLDR
It is suggested that protein kinase C regulates α12- and αz-mediated signaling pathways by preventing their association with βγ in G protein heterotrimers containing either α12 or αz.
Receptor-independent Activators of Heterotrimeric G-protein Signaling Pathways*
TLDR
AGS proteins provide unexpected mechanisms for input to heterotrimeric G-protein signaling pathways and may also serve as novel binding partners for Gα and Gβγ that allow the subunits to subserve functions that do not require initialheterotrimer formation.
Mammalian Ric-8A (Synembryn) Is a Heterotrimeric Gα Protein Guanine Nucleotide Exchange Factor*
TLDR
Purification and biochemical characterization of recombinant Ric-8A revealed that it is a potent guanine nucleotide exchange factor for a subset of Gα proteins including Gαq, Gαi1, and Gαo, but not Gαs.
GAIP, a protein that specifically interacts with the trimeric G protein G alpha i3, is a member of a protein family with a highly conserved core domain.
TLDR
Using the yeast two-hybrid system, GAIP (G Alpha Interacting Protein), that specifically interacts with the heterotrimeric GTP-binding protein G alpha i3 is identified and is likely to be an early inducible phosphoprotein.
Purification of Recombinant G Proteins from Sf9 Cells by Hexahistidine Tagging of Associated Subunits
TLDR
A method is described for purification of G protein α and β subunits from Sf9 cells infected with recombinant baculoviruses and α was characterized for the first time.
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