Identification and Characterization of a Novel Alpaca Respiratory Coronavirus Most Closely Related to the Human Coronavirus 229E

@article{Crossley2012IdentificationAC,
  title={Identification and Characterization of a Novel Alpaca Respiratory Coronavirus Most Closely Related to the Human Coronavirus 229E},
  author={Beate M. Crossley and Richard E. Mock and Scott Andrew Callison and Sharon K. Hietala},
  journal={Viruses},
  year={2012},
  volume={4},
  pages={3689 - 3700}
}
In 2007, a novel coronavirus associated with an acute respiratory disease in alpacas (Alpaca Coronavirus, ACoV) was isolated. Full-length genomic sequencing of the ACoV demonstrated the genome to be consistent with other Alphacoronaviruses. A putative additional open-reading frame was identified between the nucleocapsid gene and 3'UTR. The ACoV was genetically most similar to the common human coronavirus (HCoV) 229E with 92.2% nucleotide identity over the entire genome. A comparison of spike… 

Figures and Tables from this paper

Evidence for an Ancestral Association of Human Coronavirus 229E with Bats

The putative host switches leading to the formation of HCoV-229E were accompanied by major genomic changes, including deletions in the viral spike glycoprotein gene and loss of an open reading frame, and the evolutionary analyses show that animal and human viruses are genetically closely related, can exchange genetic material, and form a single viral species.

Rooting the Phylogenetic Tree of Middle East Respiratory Syndrome Coronavirus by Characterization of a Conspecific Virus from an African Bat

The full genome sequence of a CoV was determined directly from fecal material obtained from a South African Neoromicia capensis bat, providing evidence for the evolution of MERS-CoV in camels that preceded that in humans and suggesting that humans are infected by camels rather than vice versa.

Metagenomic Next-Generation Sequencing Reveal Presence of a Novel Ungulate Bocaparvovirus in Alpacas

An intestinal sample from an alpaca herd with reoccurring diarrhea and respiratory disease was submitted for next-generation sequencing, revealing the presence of a BoV strain and suggesting that AlBoV is a tentative new species.

Hosts and Sources of Endemic Human Coronaviruses

Link of a ubiquitous human coronavirus to dromedary camels

Although no current epidemic risk seems to emanate from these viruses, evolutionary inference suggests that the endemic human virus HCoV-229E may constitute a descendant of camelid-associated viruses.

Clinical and molecular aspects of veterinary coronaviruses

Potential Intermediate Hosts for Coronavirus Transmission: No Evidence of Clade 2c Coronaviruses in Domestic Livestock from Ghana

The emergence of Middle East Respiratory Syndrome Coronavirus (MERS-CoV), nearly a decade ago with worldwide distribution, was believed to be of zoonotic origin from bats with dromedary camels as

Coronavirus Spike Protein and Tropism Changes

Novel human coronavirus (SARS-CoV-2): A lesson from animal coronaviruses

Extensive recombination-driven coronavirus diversification expands the pool of potential pandemic pathogens

It is found that all three groups of coronaviruses with impacts on livestock or human health undergo recombination with highly diverged viruses, disrupting phylogenetic relationships and revealing contributions of unknown coronavirus lineages to the genetic diversity of established groups.
...

References

SHOWING 1-10 OF 42 REFERENCES

Analysis of the genome sequence of an alpaca coronavirus

Human coronavirus 229E encodes a single ORF4 protein between the spike and the envelope genes

The genome organization of the group 1b coronaviruses HCoV-NL63, PEDV and H coV-229E is identical, and the highly conserved amino acid sequence of the ORF4 protein among clinical isolates suggests that the protein plays an important role in vivo.

Analysis of human coronavirus 229E spike and nucleoprotein genes demonstrates genetic drift between chronologically distinct strains.

Phylogenetic analysis showed clustering of the isolates into four groups, with evidence of increasing divergence with time, and positive selection in the S gene was established, establishing two distinct viruses circulating during 2003 and 2004.

The first complete genome sequences of clinical isolates of human coronavirus 229E

The first full-length genome sequence of two clinical isolates from the cDNA-clone Inf-1 that is based on the lab-adapted strain VR-740 is presented, with a difference in genome organization: instead of an ORF4A and OrF4B, an intact ORF 4 was present in clinical isolate.

Genome structure and transcriptional regulation of human coronavirus NL63

The data show that HCoV-NL63 employs the discontinuous replication strategy with generation of subgenomic mRNAs during the (-) strand synthesis, which gives insight into the viral evolution and mutational patterns in coronaviral genome.

Identification of a Novel Coronavirus Possibly Associated with Acute Respiratory Syndrome in Alpacas (Vicugna Pacos) in California, 2007

  • B. CrossleyB. Barr R. Mock
  • Medicine
    Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
  • 2010
The findings indicate that the ARS-associated coronavirus described is distinct from the previously reported gastrointestinal-associated coroneravirus identified in alpaca herds.

PCR sequencing of the spike genes of geographically and chronologically distinct human coronaviruses 229E

Mutational Analysis of Aminopeptidase N, a Receptor for Several Group 1 Coronaviruses, Identifies Key Determinants of Viral Host Range

Enter and host range of these group 1 coronaviruses depend on the ability of the viral spike glycoproteins to recognize small, species-specific amino acid differences in the APN proteins of different species.

Feline Coronavirus Type II Strains 79-1683 and 79-1146 Originate from a Double Recombination between Feline Coronavirus Type I and Canine Coronavirus

The data show that the type II FCoVs have arisen from double recombination events: additional crossover sites were mapped in the ORF1ab frameshifting region of strain 79-1683 and in the 5′ half of ORF 1b of strains 79-1146.

Characterization of the human coronavirus 229E (HCV 229E) gene 1.

The sequence analysis of the HCV 229E gene 1 has revealed a high degree of similarity within the ORF 1b of HCV, MHV and IBV, whereas OrF 1a is much less conserved.