Identification and Characterization of a New Erythromycin Biosynthetic Gene Cluster in Actinopolyspora erythraea YIM90600, a Novel Erythronolide-Producing Halophilic Actinomycete Isolated from Salt Field

@article{Chen2014IdentificationAC,
  title={Identification and Characterization of a New Erythromycin Biosynthetic Gene Cluster in Actinopolyspora erythraea YIM90600, a Novel Erythronolide-Producing Halophilic Actinomycete Isolated from Salt Field},
  author={Dandan Chen and Junyin Feng and Lei Huang and Qinglin Zhang and Jiequn Wu and Xiangcheng Zhu and Yanwen Duan and Zhinan Xu},
  journal={PLoS ONE},
  year={2014},
  volume={9}
}
Erythromycins (Ers) are clinically potent macrolide antibiotics in treating pathogenic bacterial infections. Microorganisms capable of producing Ers, represented by Saccharopolyspora erythraea, are mainly soil-dwelling actinomycetes. So far, Actinopolyspora erythraea YIM90600, a halophilic actinomycete isolated from Baicheng salt field, is the only known Er-producing extremophile. In this study, we have reported the draft genome sequence of Ac. erythraea YIM90600, genome mining of which has… Expand
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References

SHOWING 1-10 OF 46 REFERENCES
An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea
TLDR
A novel organization of the erythromycin-producing polyketide synthase is revealed, which provides further insight into the mechanism of chain assembly. Expand
Analysis of eryBI, eryBIII and eryBVII from the erythromycin biosynthetic gene cluster in Saccharopolyspora erythraea
TLDR
Specific mutants were created in eryBI, and the resulting strains were found to synthesise erythromycin A, showing that this gene, despite its position in the biosynthetic gene cluster, is not essential for erystromycin biosynthesis. Expand
Organization of a cluster of erythromycin genes in Saccharopolyspora erythraea.
TLDR
The new Ery phenotype, EryH, was marked by the accumulation of the intermediate 6-deoxyerythronolide B (DEB), suggesting a defect in the operation of the C-6 hydroxylase system, and a block in the synthesis or addition reactions for the first sugar group. Expand
Description of the erythromycin-producing bacterium Arthrobacter sp. strain NRRL B-3381 as Aeromicrobium erythreum gen. nov., sp. nov.
TLDR
The principal long-chain fatty acids which were identified that distinguished strain NRRL B-3381T from related G + C-rich bacteria were 10-methyloctadecanoic (tuberculosteric), octadecenoic, and hexadecanoIC acids, which form the basis for the recommendation that strain NR RL B- 3381 should be the type strain of a new taxon, for which the name Aeromicrobium erythreum is proposed. Expand
Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338
TLDR
The availability of the genome sequence of S. erythraea will improve insight into its biology and facilitate rational development of strains to generate high-titer producers of clinically important antibiotics. Expand
Knockout of the Erythromycin Biosynthetic Cluster Gene, eryBI, Blocks Isoflavone Glucoside Bioconversion during Erythromycin Fermentations in Aeromicrobium erythreum but Not in Saccharopolyspora erythraea
TLDR
This study showed that isoflavone metabolism could be blocked in A. erythreum by ery BI knockout but that eryBI knockout was not sufficient to block is oflav one metabolism in S. eries, suggesting that other β-glucosidases are present. Expand
Engineering of the methylmalonyl-CoA metabolite node of Saccharopolyspora erythraea for increased erythromycin production.
Engineering of the methylmalonyl-CoA (mmCoA) metabolite node of the Saccharopolyspora erythraea wild-type strain through duplication of the mmCoA mutase (MCM) operon led to a 50% increase inExpand
Actinopolysporins A-C and tubercidin as a Pdcd4 stabilizer from the halophilic actinomycete Actinopolyspora erythraea YIM 90600.
TLDR
Four new linear polyketides were isolated from the halophilic actinomycete Actinopolyspora erythraea YIM 90600, and the structures of the new compounds were elucidated on the basis of spectroscopic data interpretation, unveiling a novel biological activity for this well-studied natural product. Expand
Sequencing and mutagenesis of genes from the erythromycin biosynthetic gene cluster of Saccharopolyspora erythraea that are involved in L-mycarose and D-desosamine production.
TLDR
From the predicted function of known eryB and eryC genes, pathways for the biosynthesis of L-mycarose and D-desosamine have been deduced. Expand
Transfer of the Type Strain of Streptomyces erythraeus (Waksman 1923) Waksman and Henrici 1948 to the Genus Saccharopolyspora Lacey and Goodfellow 1975 as Saccharopolyspora erythraea sp. nov., and Designation of a Neotype Strain for Streptomyces erythraeus
TLDR
Chemotaxonomic analysis of cells of StrePTomyces erythraeus NRRL 2338 revealed that this strain is not a representative of the genus Streptomyces because the cell walls contain meso-diaminopimelic acid, arabinose, and galactose. Expand
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