Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38).

@article{Luzio1990IdentificationSA,
  title={Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38).},
  author={J Paul Luzio and Brigitte Brake and G S Banting and Kathryn E. Howell and Paola Braghetta and Keith K. Stanley},
  journal={The Biochemical journal},
  year={1990},
  volume={270 1},
  pages={97-102}
}
Organelle-specific integral membrane proteins were identified by a novel strategy which gives rise to monospecific antibodies to these proteins as well as to the cDNA clones encoding them. A cDNA expression library was screened with a polyclonal antiserum raised against Triton X-114-extracted organelle proteins and clones were then grouped using antibodies affinity-purified on individual fusion proteins. The identification, molecular cloning and sequencing are described of a type 1 membrane… CONTINUE READING
Highly Influential
This paper has highly influenced 27 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS

Citations

Publications citing this paper.
Showing 1-10 of 115 extracted citations

Similar Papers

Loading similar papers…