Identification, purification and reconstitution of thiamin metabolizing enzymes in human red blood cells.

@article{Egi1992IdentificationPA,
  title={Identification, purification and reconstitution of thiamin metabolizing enzymes in human red blood cells.},
  author={Yuichiro Egi and Shinya Koyama and Tetsuji Shioda and Kazuko Yamada and Toshisuke Kawasaki},
  journal={Biochimica et biophysica acta},
  year={1992},
  volume={1160 2},
  pages={171-8}
}
Thiamin and its mono- (TMP), di- (TDP) and triphosphate (TTP) were assayed in adult human whole blood using high-performance liquid chromatography (HPLC). TDP and TTP were detected in red blood cells (RBC), but not in plasma. After incubation with 20 microM thiamin and 5 mM glucose for 2 h, the TDP and TTP contents of RBC increased from 111 to 222 and 0.6 to 2.2 nmol/l of packed RBC, respectively, suggesting enzymatic conversion of thiamin to TDP and then to TTP. Thiamin pyrophosphokinase (TPK… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-2 of 2 extracted citations

Similar Papers

Loading similar papers…