Identification, production, and use of polyol-responsive monoclonal antibodies for immunoaffinity chromatography.

@article{Thompson2009IdentificationPA,
  title={Identification, production, and use of polyol-responsive monoclonal antibodies for immunoaffinity chromatography.},
  author={Nancy E. Thompson and Katherine M Foley and Elizabeth S Stalder and R. R. Burgess},
  journal={Methods in enzymology},
  year={2009},
  volume={463},
  pages={475-94}
}
Immunoaffinity chromatography is a powerful tool for purification of proteins and protein complexes. The availability of monoclonal antibodies (mAbs) has revolutionized the field of immunoaffinity chromatography by providing a continuous supply of highly uniform antibody. Before the availability of mAbs, the recovery of the target protein from immobilized polyclonal antibodies usually required very harsh, often denaturing conditions. Although harsh conditions are often still used to disrupt the… CONTINUE READING

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