Identification, cloning and expression analysis of the pluripotency promoting Nanog genes in mouse and human

@article{Hart2004IdentificationCA,
  title={Identification, cloning and expression analysis of the pluripotency promoting Nanog genes in mouse and human},
  author={A. Hart and L. Hartley and M. Ibrahim and L. Robb},
  journal={Developmental Dynamics},
  year={2004},
  volume={230}
}
The murine Nanog gene, a member of the homeobox family of DNA binding transcription factors, has been shown recently to maintain pluripotency of embryonic stem cells. We have used a sequence homology and expression screen to identify and clone the mouse and human Nanog genes and characterized their phylogenetic context and expression patterns. We report here the gene structure and expression patterns of the mouse Nanog gene, the human Nanog and Nanog2 genes, and six processed human Nanog… Expand
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TLDR
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The findings suggest that NANOG acts as a gatekeeper of pluripotency in human embryonic stem and carcinoma cells by preventing their differentiation to extraembryonic endoderm and trophectoderm lineages. Expand
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Several aspects of the structure, expression and function of the NANOG gene are highlighted, including also its role in tumourigenesis and various types of cancers, cancer prognosis and anticancer therapies. Expand
Nanog regulates Pou3f1 expression and represses anterior fate at the exit from pluripotency
TLDR
A previously unknown mechanism by which Nanog specifically represses the anterior epiblast lineage is unveiled and an active role of Nanog inhibiting the neural fate by repressing Pou3f1 at the onset of gastrulation is indicated. Expand
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TLDR
The GNP-EGFP reporter system represents a useful tool to monitor endogenous NANOG activation and for research with goat pluripotent stem cells. Expand
Nanog regulates Pou3f1 expression at the exit from pluripotency during gastrulation
TLDR
Nanog is a key factor for pluripotency, but it has further roles during gastrulation by regulating lineage determinants such as Pou3f1, which indicates an active role of Nanog inhibiting neural regulatory networks by repressing Pou2f1 at the onset of gastrulations. Expand
Functional characterization of NANOG in goat pre-implantation embryonic development.
TLDR
Analysis of expression of developmentally important genes showed that NANOG knockdown results in significant increase in expression of SOX2 and OCT4 and among the possible target genes (CDX2, REX1 and GATA4) of this network, only GATA 4 showed increased expression. Expand
NANOG Reporter Cell Lines Generated by Gene Targeting in Human Embryonic Stem Cells
TLDR
The newly derived NANOG reporter hESC lines present novel tools to visualize NanOG expression in viable hESCs and can be used to elucidate the function and regulation of NANog in pluripotent hEScs. Expand
The pluripotency factor NANOG controls primitive hematopoiesis and directly regulates Tal1
TLDR
The results show that Nanog regulates primitive hematopoiesis by directly repressing critical erythroid lineage specifiers, and Nanog‐deficient embryonic stem cells are prone to erythropoietic differentiation. Expand
A Distinct Expression Pattern in Mammalian Testes Indicates a Conserved Role for NANOG in Spermatogenesis
TLDR
A conserved role for NANOG is suggested in meiotic and post-meiotic stages of male germ cell development and in the first steps of haploid germ cell maturation (spermiogenesis). Expand
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