Identification, cloning, and characterization of microsatellite DNA in Euglena gracilis.

  title={Identification, cloning, and characterization of microsatellite DNA in Euglena gracilis.},
  author={Wen-jing Zhang and Jun Yang and Yu-He Yu and Y Shen},
  journal={The Journal of eukaryotic microbiology},
  volume={52 4},
Microsatellite DNA has been developed into one of the most popular genetic markers. We have identified and cloned microsatellite loci in the genome of a free-living protozoan Euglena gracilis FACHB-848, using the random amplified microsatellites method (RAMS). The digoxigenin-labelled oligonucleotides (CT)10 and (GT)10 served as probes to detect complementary sequences in the randomly amplified polymorphic DNA (RAPD) fingerprints produced by means of Southern blotting. Subsequently, positive… CONTINUE READING


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