Identification, characterization and molecular adaptation of class I redox systems for the production of hydroxylated diterpenoids

@article{Grner2016IdentificationCA,
  title={Identification, characterization and molecular adaptation of class I redox systems for the production of hydroxylated diterpenoids},
  author={Christian G{\"o}rner and Patrick Schrepfer and Veronika Redai and Frank H. Wallrapp and Bernhard Loll and Wolfgang Eisenreich and Martin Haslbeck and Thomas B. Br{\"u}ck},
  journal={Microbial Cell Factories},
  year={2016},
  volume={15}
}
BackgroundDe novo production of multi-hydroxylated diterpenoids is challenging due to the lack of efficient redox systems.ResultsIn this study a new reductase/ferredoxin system from Streptomyces afghaniensis (AfR·Afx) was identified, which allowed the Escherichia coli-based production of the trihydroxylated diterpene cyclooctatin, a potent inhibitor of human lysophospholipase. This production system provides a 43-fold increase in cyclooctatin yield (15 mg/L) compared to the native producer. AfR… 
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